Jet InjectorActranza^® lab.> See in Japanese

Product Profiles

Product Features

• Optimized for administration in small animals (mice, rats, etc.) with compatibility to medium animals
• Intradermal, sub-cutaneous, and intramuscular administration
• Minimally-invasive injections without needles
• Achieves transfection without formulation, LNPs, or carriers
• Ideal for antibody generation, gene expression, and vaccine research

Actranza^® lab. as a Drug Delivery System

When the jet stream is released, it could generate shear stress within cells, which may enhance cell membrane permeability or promoting endocytosis.
As a result, DNA or mRNA could be delivered directly into cells without the need for carriers such as lipid nanoparticles (LNPs) or viral vectors.

Applications

• 

  Drug Distribution

  The liquid disperses over a wide area in intradermal tissue (India ink).

• 

  Efficient Delivery of Plasmid DNA into Cells

  This shows that the fluorescently labeled (Cy^™3) plasmid DNA is efficiently taken up into the cells nucleus (pink fluorescent granules indicate the introduction of plasmid DNA into the cell nucleus).
  ^※Cy^™ is a trademark of Cytiva.

• 

  GFP gene expression

  Expression of the gene encoding Green Fluorescence Proteins (GFP) by injection.

• 

  LacZ gene expression

  Expression of the gene encoding β-galactosidase by injection of plasmid DNA (blue areas are expression sites).

Overview of Components & Functions

• 1Control Unit

  

  • Easy-to-grip
  • Reusable
  • Safety mechanism to prevent accidental injection

• 2Actuator

  

  • Available in models for mice or rats
  • Cartridge-type
  • Single use

• 3Container Unit

  

  • Includes loading adaptor
  • Electron beam irradiated
  • Single use

Product specifications

Product lineup

Note: The names of container units are based on their capacity and do not indicate the exact delivery volume.

• This device is for experimental animal studies only.
• This device is not a medical device nor its accessory, which is not intended for any other purposes including the diagnosis, therapeutic intervention or prevent of disease in human or animals.
• Product specifications are subject to change without prior notice.

Publications

• A new, patient-friendly, needleless methotrexate administration method using the pyro-drive jet injector in rats, Mikiko Shimizu et al., Scientific Reports. 2025 November 12;15(1):39599.
  https://doi.org/10.1038/s41598-025-23302-6
• Intradermal Injection of a Protein Alone Without Additional Adjuvants Using a Needle-Free Pyro-Drive Jet Injector Induces Potent CD8+ T Cell-Mediated Antitumor Immunity, Jukito Sonoda et al., International Journal of Molecular Sciences. 2025 May 7;26(9):4442.
  https://doi.org/10.3390/ijms26094442
• Carrier-free mRNA vaccine induces robust immunity against SARS-CoV-2 in mice and non-human primates without systemic reactogenicity, Saed Abbasi et al., Molecular Therapy. 2024 May 1;32(5):1266-1283.
  https://doi.org/10.1016/j.ymthe.2024.03.022
• Induction of potent antitumor immunity by intradermal DNA injection using a novel needle-free pyro-drive jet injector, Shinya Inoue et al., Cancer Science, Volume114, Issue1, January 2023, Pages 34-47.
  https://doi.org/10.1111/cas.15542
• Phase I Study to Assess the Safety and Immunogenicity of an Intradermal COVID-19 DNA Vaccine Administered Using a Pyro-Drive Jet Injector in Healthy Adults, Hironori Nakagami et al., Vaccines 2022, 10(9), 1427.
  https://doi.org/10.3390/vaccines10091427
• Immune response induced in rodents by anti-CoVid19 plasmid DNA vaccine via pyro-drive jet injector inoculation, Tomoyuki Nishikawa et al., Immunological Medicine, Volume 45, 2022 - Issue 4, Pages 251-264.
  https://doi.org/10.1080/25785826.2022.2111905
• Mechanism of jet injector-induced plasmid DNA uptake: Contribution of shear stress and endocytosis, Hiroshi Miyazaki et al., International Journal of Pharmaceutics. Volume 609, 20 November 2021, 121200.
  https://doi.org/10.1016/j.ijpharm.2021.121200
• “Section 6: Development of a novel needle-free device to administer DNA vaccines”, Developments in immunology and their applications that every researcher in industry and academia should know, Hiromitsu Iga, Takamasa Suzuki, Hiroshi Miyazaki, (Japan, Technical Information Institute Co. LTD, 2021)
• Gene transfer by pyro-drive jet injector is a novel therapeutic approach for muscle diseases,Takafumi Nakae et al., Gene. Volume 788, 1 July 2021, 145664.
  https://doi.org/10.1016/j.gene.2021.145664
• Anti-CoVid19 plasmid DNA vaccine induces a potent immune response in rodents by Pyro-drive Jet Injector intradermal inoculation, Tomoyuki Nishikawa et al., bioRxiv, Posted January 14, 2021.
  https://doi.org/10.1101/2021.01.13.426436
• Stable Immune Response Induced by Intradermal DNA Vaccination by a Novel Needleless Pyro-Drive Jet Injector, C.Chang et al., AAPS PharmSciTech. 21, Article number: 19 (2020) .
  https://doi.org/10.1208/s12249-019-1564-z
• Development of Pyro-Drive Jet Injector With Controllable Jet Pressure, H.Miyazaki et al., Journal of Pharmaceutical Sciences. 2019 Jul;108(7): 2415-2420.
  https://doi.org/10.1016/j.xphs.2019.02.021
• Estimation of High-Speed Liquid-Jet Velocity Using a Pyro Jet Injector, Naohisa Takagaki et al., Scientific Reports Rep 9, 19859 (2019).
  https://doi.org/10.1038/s41598-019-56511-x

Related News

• Apr. 15, 2024

  Lecture by Prof. Satoshi Uchida, Tokyo Medical and Dental University at the World Vaccine Congress 2024, USA Research on mRNA vaccines using DAICEL's "Actranza^® lab."
  https://www.daicel.com/en/news/2024/20240415_959.html

• Apr. 22, 2022

  DAICEL provides Immunetune with "Actranza^™ lab. Technology" for their development of next generation DNA vaccines against cancer and infection diseases
  https://www.daicel.com/en/news/assets/pdf/20220422_2.pdf

• Jan. 28, 2019

  Daicel Developed Pyro-drive Jet Injector "Actranza^™ lab." for Intracellular Drug Delivery in the Laboratory
  https://www.daicel.com/en/news/2019/20190128_290.html

Trademark Attribution

Actranza^® lab. is a registered trademark of Daicel Corporation in Japan and other countries.